示例
示例1
稀释待检患者血清:取一中试管，加生理盐水 3.8ml 和患者血清 0.2ml，充分混匀， 此时血清稀释度为1:20，吸此血清 2ml 分别加入每排的第1管中，每管0.5ml。此时中试管内剩余稀释血清2ml，再加入生理盐水2ml，使之稀释成1:40。再加入每排的第2管中，每管0.5ml。以此类推，将中试管内剩余血清依次作倍比稀释，并依次将稀释血清加至每排第3 至7管中，则每排各管的血清稀释度 1:20、1:40、1:80、1:160、1:320、1:640、1:1280。毎排第8管不加血清，只加0.5ml 生理盐水作为对照。
示例2
取经处理并已作1:5预稀释的患者血清0.2ml，加入第1管中作1:10稀释，吹打3 次混匀，取0.2ml 加至第2管，并依次作倍比稀释，到第8管止。从第8管中取出0.2ml 弃至消毒缸内。第9管为病毒对照，不加血清;第10管为血清对照，直接加人0.2ml 1:5稀释的待测血清，不加病毒液。
操作要点
- 第一管稀释为浓度最高的
- 每管稀释液的量是一样的
- 根据每管稀释液的量和稀释倍数决定管间转移的量
- 每管的液体量应适当大于所需？考虑损耗，在枪头上的残留等等
- 预润洗移液器吸头
- Determine the diluent: First of all, you need to choose the proper diluent for the substance that you want to dilute. Many compounds can be mixed with distilled water, but for bacteria or cells, for example, a fresh culture medium should be used.
确定稀释剂：首先，您需要为您要稀释的物质选择合适的稀释剂。许多化合物可以与蒸馏水混合，但对于细菌或细胞，例如，应使用新鲜的培养基。 - Fill target containers with diluent: Once you have determined the diluent, fill the tubes or wells that you will use to set up the serial dilution with the desired amount of diluent (Figure 3, Step 1). More information on how to calculate the diluent volume can be found further below.
用稀释剂填充目标容器：确定稀释剂后，用所需量的稀释剂填充将用于设置连续稀释的管或孔（图 3，步骤 1）。有关如何计算稀释剂体积的更多信息，请参见下文。 - Perform the first dilution: Ensure that the sample, reagent, chemical or compound that you want to dilute is well mixed, then transfer a defined volume to the first tube or well (Figure 3, Step 2). If you don't know what the required transfer volume for your experiment should be, please refer to the section 'Serial dilution calculations'.
执行第一次稀释：确保要稀释的样品、试剂、化学品或化合物充分混合，然后将规定体积转移到第一个管或孔中（图 3，步骤 2）。如果您不知道实验所需的转移体积是多少，请参阅“连续稀释计算”部分。 - Perform the second dilution: Thoroughly mix the first dilution (Figure 3, Step 3), then aspirate the same transfer volume that you used in the previous step, and dispense it into the second tube or well (Figure 3, Step 4).
执行第二次稀释：彻底混合第一次稀释（图 3，步骤 3），然后吸出与上一步中使用的相同的转移体积，并将其分配到第二个管或孔中（图 3，步骤 4）。 - Repeat: Extend the process of mixing the most recently obtained dilution and transferring some of it to the next tube or well, until you get to the end of your serial dilution experiment (Figure 3, Steps 5-11).
重复：延长混合最近获得的稀释液并将其中一些转移到下一个管或孔中的过程，直到完成连续稀释实验（图 3，步骤 5-11）。 - Discard transfer volume: Your last tube or well will contain more liquid than all the previous dilutions. For some applications, this is not an issue at all. For example, when you set up a serial dilution of a bacterial culture in tubes and want to incubate 1 ml of every tube with culture media. In some cases, however, the liquid volume in every tube or well needs to be equal, such as when your plate needs to go into a plate reader for analysis. In this case, aspirate the transfer volume from the last tube or well and discard it at the end of setting up your serial dilution (Figure 4, Step 12).
丢弃转移体积：您的最后一个管或孔将包含比之前所有稀释液更多的液体。对于某些应用程序来说，这根本不是问题。例如，当您在试管中设置细菌培养物的连续稀释并希望用培养基孵育每个试管中的 1 ml 时。然而，在某些情况下，每个管或孔中的液体体积需要相等，例如当您的板需要进入读板器进行分析时。在这种情况下，从最后一个管或孔中吸出转移体积，并在设置连续稀释结束时将其丢弃（图 4，步骤 12）。
